Figure 2. IL-10 inhibits transcription elongation at TNF. (A and B) Cells were treated with LPS with or without IL-10 for 30 min followed by ChIP using Pol II, phospho-Ser2–specific, or rabbit isotype control antibodies at the TSS or downstream regions of the TNF (A) or NFKBIA (B) genes. (C) Cells were left unstimulated or preincubated for 30 min with 500 nM flavopiridol, followed by LPS stimulation for 30 min, and then subjected to ChIP. (D) ChIP assay of CDK9 recruitment to the start of the TNF or NFKBIA genes upon stimulation with LPS with or without IL-10. Data are expressed as mean ± SD of triplicate measurements for a single donor, representative of three independent experiments using different donors.
Figure 2.

IL-10 inhibits transcription elongation at TNF. (A and B) Cells were treated with LPS with or without IL-10 for 30 min followed by ChIP using Pol II, phospho-Ser2–specific, or rabbit isotype control antibodies at the TSS or downstream regions of the TNF (A) or NFKBIA (B) genes. (C) Cells were left unstimulated or preincubated for 30 min with 500 nM flavopiridol, followed by LPS stimulation for 30 min, and then subjected to ChIP. (D) ChIP assay of CDK9 recruitment to the start of the TNF or NFKBIA genes upon stimulation with LPS with or without IL-10. Data are expressed as mean ± SD of triplicate measurements for a single donor, representative of three independent experiments using different donors.

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