Figure 3.
![Figure 3. Effect of elevated [K+]o and salbutamol on force development in mouse soleus. Muscles from 12.5-wk-old WT and mutant mice were mounted in force transducers for isometric contractions in standard KR buffer and stimulated as indicated every 10 min using 2-s trains of 120-Hz pulses of 0.2-ms duration and 12 V. After 60 min, the buffer was changed to a KR in which K+ was increased by 6 mM and Na+ was decreased by 6 mM to maintain isoosmolarity. After 50 min, 10−6 M salbutamol was added to all muscles, and stimulation every 10 min continued. Each point represents the mean of observations on four muscles, with error bars denoting SEM.](https://cdn.rupress.org/rup/content_public/journal/jgp/138/1/10.1085_jgp.201010586/4/jgp_201010586_lw_fig3.jpeg?Expires=1767726441&Signature=znqzOC-3R7elvUxN3LzhcKjDVmdtSN~QRLZcKOYwNkXExUqobEjNpN4qSbz~o4vpxjD4DTIe1woXMKV8AO2WQiCEhNzuW2awV559lCigqZjKSiZytznqi8Sl6pzh1g0~kbpj~u7XFMnhZ5CBVBjwcE~ry9oXphkBxZczjy5AUWzSqDYSxSYlOvP6-KynDEDnLNVhWSQsZMDbTtwTM~1Sa1ifnX53MT6bANU0n54JQApV2HkZckhlladbHYZzkf0~F6TVxt32m0d2lFuBtOnHr8Kyem8pbQYycrK3eccnJrijFnPeEv9wbnO60ZPRVLPnQK0mlqvqomSi~eL~4q6sig__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of elevated [K+]o and salbutamol on force development in mouse soleus. Muscles from 12.5-wk-old WT and mutant mice were mounted in force transducers for isometric contractions in standard KR buffer and stimulated as indicated every 10 min using 2-s trains of 120-Hz pulses of 0.2-ms duration and 12 V. After 60 min, the buffer was changed to a KR in which K+ was increased by 6 mM and Na+ was decreased by 6 mM to maintain isoosmolarity. After 50 min, 10−6 M salbutamol was added to all muscles, and stimulation every 10 min continued. Each point represents the mean of observations on four muscles, with error bars denoting SEM.
