Examples for fitting of amplitude histograms in the presence of 0.3 mM enrofloxacin on the cis side in an LPS membrane. Data are taken from Fig. 4 B. (A) Successful fitting of Gaussians (red) to an all-point amplitude histogram with slow blocking measured at −100 mV resulting in σ₀ = 2.6 pA and an estimated single-pore current I_app = 20.8 pA. (B) Failure of fitting an open-point histogram measured at +100 mV by a Gaussian distribution (blue dotted line). Successful fitting of the data with a β distribution (red solid line) resulting in I_true = 22.7 pA, I_B = 12 pA, k_OB = 20 700 s^-1, and k_BO = 67 000 s^-1 (Eq. 1, Figs. 1 C and 5).