Figure 6.
![Figure 6. TMEM16B mutations. (A) Predicted topology of TMEM16A and TMEM16B from hydropathy analysis. (B) Alignment between mouse TMEM16A (a,c; available from GenBank/EMBL/DDBJ under accession no. NM_178642.4) and the retinal isoform of TMEM16B used in this study (NP_705817.1), with the mutations or deletions highlighted in color. (C–F) Representative recordings at the indicated [Ca2+]i for E367Q (C), ΔE5 (D), ΔEYE (E) mutants, and WT (F). Traces for WT are the same as in Fig. 2 A. Voltage protocol as in Fig. 1 A. (G) I-V steady-state relations (n = 3–8). (H) Average ratios between currents measured at +100 and −100 mV plotted versus [Ca2+]i for each mutant (n = 3–8).](https://cdn.rupress.org/rup/content_public/journal/jgp/139/4/10.1085_jgp.201110764/4/jgp_201110764_fig6.jpeg?Expires=1767712028&Signature=t~yQaCQO2QaVSyxhYc0dXYkMSDFCXCi3DbLQaKhW02eMonZ5HGMmnXl438eSU29lobNqqHEPAq3jhhucJ0qAABy9b-vqiA~7j6acHuIztjQLueDREs152zEhE5CTaFW~49tjFuZnBu7c0r~zPvwR82TPncJPFu0xIkBJmgDgazcUA78gna1nCuGunXs4dvEDCa7qh~F0m-xIElw9DITXjrHeXpJGc5eeUYLYYP9isyz6-f1C3htcrRN9q1foPQNvg~PCrTqoOzhkDmnQN1YsSHX0sQtBChZjX0HtPq7s9HbpM9bNHtukhfjTXRrGv3w6hjzKncWMLN78yyVIaaaxKA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TMEM16B mutations. (A) Predicted topology of TMEM16A and TMEM16B from hydropathy analysis. (B) Alignment between mouse TMEM16A (a,c; available from GenBank/EMBL/DDBJ under accession no. NM_178642.4) and the retinal isoform of TMEM16B used in this study (NP_705817.1), with the mutations or deletions highlighted in color. (C–F) Representative recordings at the indicated [Ca2+]i for E367Q (C), ΔE5 (D), ΔEYE (E) mutants, and WT (F). Traces for WT are the same as in Fig. 2 A. Voltage protocol as in Fig. 1 A. (G) I-V steady-state relations (n = 3–8). (H) Average ratios between currents measured at +100 and −100 mV plotted versus [Ca2+]i for each mutant (n = 3–8).
