Comparison of TEA inhibition between Kcv and substitutions L70Y and L70A. 150 mM KCl buffered with 10 mM Tris, pH 7.2. (A) IC₅₀ at various voltages for wt-Kcv, cis TEA (closed circles); L70Y, cis TEA (closed boxes); L70A, cis TEA (closed triangles); wt-Kcv, trans TEA (open circles); L70Y, trans TEA (open boxes); and L70A, trans TEA (open triangles). (B) Vertical bar comparison of TEA sensitivity from the cis and trans sides at ±60 mV for wt-Kcv, L70Y, and L70A. Data points are presented as mean ± SD.