Inhibition of the wt-Kcv K⁺ channel by TEA. Single-channel currents were recorded in symmetric solutions containing 150 mM KCl buffered with 10 mM Tris, pH 7.2. (A and B) Current traces in the presence of different TEA concentrations added from the cis solution (A) and trans solution (B) at −60 mV (left) and 60 mV (right). (C) Inhibition percentage (I/I₀) in various TEA concentrations measured from traces shown in A and B. Closed circles indicate cis TEA, −60 mV; closed boxes indicate cis TEA, 60 mV; open circles indicate trans TEA, −60 mV; open boxes indicate trans TEA, 60 mV. Data are presented as mean ± SD.