Figure 3.
![Figure 3. Di-8-ANEPPS transients and currents recorded before and after blocking ICl. (A) Single sweep di-8-ANEPPS records obtained in a fiber with 40 mM [Cl]i under control conditions. Pulses (50-ms rectangular steps) ranging in amplitude from −100 to +100 mV were applied every 20 mV from a VH = −40 mV (trace colors: cyan, red, violet, blue, purple, olive, pink, dark blue, brown, orange, and gray, respectively). (B) Di-8-ANEPPS transients recorded in response to the same pulses as in A, but in the presence of 9-ACA. (C) Ionic current records detected simultaneously with the transients in A. (D) Ionic current records detected simultaneously with the transients in B. Same fiber as in Fig. 1 A.](https://cdn.rupress.org/rup/content_public/journal/jgp/137/1/10.1085_jgp.201010496/5/jgp_201010496_rgb_fig3.jpeg?Expires=1767805651&Signature=A3SgivwXJX2rfkYZCwEOIxof72maEsn9MPjx6YSLAv4o1vpuW7dnFcYgEboIqLLL3hRWSuVPfPBunnvafJ2SkNWk12MwpkT9qjzaPc~4GOHOwYpiNCrziWzBPGhLXzsDMruN60g3yXy3W5U4pp65zNLaq9y6cydBejSMMGhaWoXRFOxmStBdhfWzyWpa2j4IcwufAlYnhtyuq-uN0KywekJriltfP-gDy19pJgzIhxW4sYf-hMvcvAmkFVf00LksAp2Y5PzrbKESNiGZ~aU54BwIbMitdwMZYKxDuSHPEJz8VUmGdc-fK8Mz8QwVj7ldIPeSYSDYJOAw78BxwxbaVA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Di-8-ANEPPS transients and currents recorded before and after blocking ICl. (A) Single sweep di-8-ANEPPS records obtained in a fiber with 40 mM [Cl]i under control conditions. Pulses (50-ms rectangular steps) ranging in amplitude from −100 to +100 mV were applied every 20 mV from a VH = −40 mV (trace colors: cyan, red, violet, blue, purple, olive, pink, dark blue, brown, orange, and gray, respectively). (B) Di-8-ANEPPS transients recorded in response to the same pulses as in A, but in the presence of 9-ACA. (C) Ionic current records detected simultaneously with the transients in A. (D) Ionic current records detected simultaneously with the transients in B. Same fiber as in Fig. 1 A.
