![Figure 2. Currents elicited by single pulses in a fiber equilibrated with 40 mM [Cl]i. (A) Total ionic currents recorded in TEA-external. The inset shows the corresponding membrane potential records. (B) Residual currents in the presence of 0.5 mM 9-ACA. The inset shows the corresponding membrane potential records. (C) 9-ACA–sensitive currents obtained by one-to-one subtraction of the records in B from those in A. In A–C, pulses ranged from –120 to +120 mV, applied every 20 mV from a VH = –40 mV. Trace color sequence: black (–120 mV), cyan, red, violet, blue, purple, olive, pink, dark blue, brown, orange, gray, and dark yellow (120 mV). (D) Voltage dependence of peak and steady-state values of 9-ACA–sensitive currents (ICl-peak and ICl-ss, black and red symbols, respectively). The steady-state values of the 9-ACA–insensitive currents are plotted in blue symbols. The arrow indicates VH. Fiber parameters: radius = 26 μm; length = 364 μm; capacitance: 4.9 μF/cm2.](https://cdn.rupress.org/rup/content_public/journal/jgp/137/1/10.1085_jgp.201010496/5/jgp_201010496_rgb_fig2.jpeg?Expires=1767735894&Signature=oaYzK4ln-lNo04tq6UrsnlCCZiH2gigcMz2jQkQlclx0q-BbMCoVtWUf8qJaEZAOwkME7youxFf1~6sQ30GWcYUZyX4K63VicbsgXo8X5m1p9o4deqK~PNv1bBgXQJWZUAn3rPbWkfwFHlZQTbt4pOaZnn82zvoCwmMQaoEP1BF2gcwOTiHTjYP3PMowqTsT2QAdRg0dWcmbKQxv0D1hFCqI2tgA8YvQ5aGHPm065i56K0EgvhTgDNkOBKscp-Ul10BZve7Top5KoefVwI4WUfeO95md7KXAhKVvd6W62zi6lKEeNQxue1n6Ui48cPtxG6xXUEqIswrJJ~6xYFRgDw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Currents elicited by single pulses in a fiber equilibrated with 40 mM [Cl]i. (A) Total ionic currents recorded in TEA-external. The inset shows the corresponding membrane potential records. (B) Residual currents in the presence of 0.5 mM 9-ACA. The inset shows the corresponding membrane potential records. (C) 9-ACA–sensitive currents obtained by one-to-one subtraction of the records in B from those in A. In A–C, pulses ranged from –120 to +120 mV, applied every 20 mV from a VH = –40 mV. Trace color sequence: black (–120 mV), cyan, red, violet, blue, purple, olive, pink, dark blue, brown, orange, gray, and dark yellow (120 mV). (D) Voltage dependence of peak and steady-state values of 9-ACA–sensitive currents (ICl-peak and ICl-ss, black and red symbols, respectively). The steady-state values of the 9-ACA–insensitive currents are plotted in blue symbols. The arrow indicates VH. Fiber parameters: radius = 26 μm; length = 364 μm; capacitance: 4.9 μF/cm2.
