Figure 8.
Figure 8. The human KCNQ1 F127V mutant confirms that the S1 segment of KCNQ1 is important for the KCNE3 modulation. (A) Representative current traces for the hKCNQ1 F127V mutant in the absence (left) and presence (right) of KCNE3. The membrane potential was stepped up from −100 to +60 mV in 20-mV increments. (B) G-V relationships for the hQ1 F127V mutant in the absence (closed circles) and presence of KCNE1 (red squares) or KCNE3 (blue triangles). The G-V relationships of WT hKCNQ1 in the absence (black dotted curve) and presence of KCNE1 (red dotted curve) or KCNE3 (blue dotted curve) are superimposed. (C) Representative current traces for the hKCNQ1 F127V mutant in the presence of KCNE3 from the same oocyte at different holding potentials (−80 and −120 mV). 2-s depolarizing pulses were applied at every 10 s. (D) G-V relationships for the hQ1 F127V mutant with KCNE3 at a holding potential of −80 mV (open circles) and −120 mV (closed circles).

The human KCNQ1 F127V mutant confirms that the S1 segment of KCNQ1 is important for the KCNE3 modulation. (A) Representative current traces for the hKCNQ1 F127V mutant in the absence (left) and presence (right) of KCNE3. The membrane potential was stepped up from −100 to +60 mV in 20-mV increments. (B) G-V relationships for the hQ1 F127V mutant in the absence (closed circles) and presence of KCNE1 (red squares) or KCNE3 (blue triangles). The G-V relationships of WT hKCNQ1 in the absence (black dotted curve) and presence of KCNE1 (red dotted curve) or KCNE3 (blue dotted curve) are superimposed. (C) Representative current traces for the hKCNQ1 F127V mutant in the presence of KCNE3 from the same oocyte at different holding potentials (−80 and −120 mV). 2-s depolarizing pulses were applied at every 10 s. (D) G-V relationships for the hQ1 F127V mutant with KCNE3 at a holding potential of −80 mV (open circles) and −120 mV (closed circles).

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