Figure 7.
Figure 7. The V46F mutation with a human S5P connector changes Ci-KCNQ1 into a KCNE3-sensitive channel. (A) The constitutive activity index (G−80mV/G+20mV) with KCNE3 for the point mutants of the ciQ1hQ1(S2–S3,S5P) chimera. Asterisks indicate the significant increase in the level of G−80mV/G+20mV, with KCNE3 compared with that of ciQ1hQ1(S2–S3,S5P) with KCNE3 (Dunnett’s test). The locations of the mutations are indicated by yellow stars in the inset. (B) Representative current traces of ciQ1hQ1(S5P) V46F in the absence and presence of KCNE3. The membrane potential was stepped up from −100 to +60 mV and subsequently stepped to −30 mV for tail currents. (C) G-V relationships for Ci-KCNQ1 V46F and ciQ1hQ1(S5P) V46F in the absence (open symbols) and presence (closed symbols) of KCNE3. Plots are fitted with the Boltzmann equation.

The V46F mutation with a human S5P connector changes Ci-KCNQ1 into a KCNE3-sensitive channel. (A) The constitutive activity index (G−80mV/G+20mV) with KCNE3 for the point mutants of the ciQ1hQ1(S2–S3,S5P) chimera. Asterisks indicate the significant increase in the level of G−80mV/G+20mV, with KCNE3 compared with that of ciQ1hQ1(S2–S3,S5P) with KCNE3 (Dunnett’s test). The locations of the mutations are indicated by yellow stars in the inset. (B) Representative current traces of ciQ1hQ1(S5P) V46F in the absence and presence of KCNE3. The membrane potential was stepped up from −100 to +60 mV and subsequently stepped to −30 mV for tail currents. (C) G-V relationships for Ci-KCNQ1 V46F and ciQ1hQ1(S5P) V46F in the absence (open symbols) and presence (closed symbols) of KCNE3. Plots are fitted with the Boltzmann equation.

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