Effect of TsVII on rNav1.8. (A) rNav1.8 currents at +15 mV are not affected by 100 nM TsVII (holding voltage was −90 mV). (B) G-V relationship of rNav1.8 before (black) and after (green) the application of 100 nM TsVII. n = 3, and error bars represent SEM. (C) rNav1.8 currents at +15 mV are only slightly inhibited when 1 µM TsVII is applied in combination with a preceding 5-ms depolarizing pulse to +30 mV (holding voltage was −90 mV) (Cestèle et al., 1998). (D) Deduced G-V relationship of rNav1.8 before (black) and after (green) the application of 100 nM TsVII. Open circles indicate the use of a depolarizing prepulse. n = 3, and error bars represent SEM.