Intermolecular CALI in KR/AQP4 chimeras. (A, left) Schematic of cells expressing KR/AQP4 chimeras together with unconjugated AQP1. (A, right) AQP4 or AQP1 immunoblot after BN/PAGE of lysates from cells expressing M1-AQP4-KR or M23-AQP4-KR, together with unconjugated AQP1, with and without CALI. (B, left) Schematic of cells expressing M23-AQP4-KR together with myc-tagged M23-AQP4. (A, right) AQP4 and myc immunoblot of BN/PAGE of lysates from cells expressing M23-AQP4-KR together with myc-M23-AQP4, with and without CALI. Gels that are apart were run separately. (C) Schematic of distance and clustering-dependent CALI in M1-AQP4 and in OAP-forming M23-AQP4.