Figure 5. CALI reduces binding of NMO-IgG to an AQP4/KR chimera. (left) Schematic showing CALI of cells expressing M23-AQP4-KR and labeled with NMO-IgG and a secondary Alexa Fluor 488 anti–human antibody. (top middle) CALI (exposure to red light for 60 s) was performed in the indicated circles on cells expressing M23-AQP4-KR and labeled with NMO-IgG and a secondary Alexa Fluor 488 anti–human antibody. (bottom middle) CALI of control cells expressing cytoplasmic KR and unconjugated M23-AQP4. (right) Quantification of KR bleaching and loss of green Alexa Fluor 488 fluorescence after CALI (n = 4, error bars indicate SE; *, P < 0.01).
Figure 5.

CALI reduces binding of NMO-IgG to an AQP4/KR chimera. (left) Schematic showing CALI of cells expressing M23-AQP4-KR and labeled with NMO-IgG and a secondary Alexa Fluor 488 anti–human antibody. (top middle) CALI (exposure to red light for 60 s) was performed in the indicated circles on cells expressing M23-AQP4-KR and labeled with NMO-IgG and a secondary Alexa Fluor 488 anti–human antibody. (bottom middle) CALI of control cells expressing cytoplasmic KR and unconjugated M23-AQP4. (right) Quantification of KR bleaching and loss of green Alexa Fluor 488 fluorescence after CALI (n = 4, error bars indicate SE; *, P < 0.01).

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