Figure 4. CALI efficiency depends on intermolecular topography. FRT cells were transfected with YFP alone or together with M1-AQP4-KR or M23-AQP4-KR. (A, left) AQP4 immunoblot after BN/PAGE. Gels that are apart were run separately. (A, right) TIRFM showing OAP formation by M23-AQP4-KR but not by M1-AQP4-KR. (B) Osmotic water permeability measured by YFP fluorescence, as in Fig. 3 B. (C) Relative osmotic water permeabilities (error bars indicate SE, n = 5–8 cells on three cover glasses; *, P < 0.01).
Figure 4.

CALI efficiency depends on intermolecular topography. FRT cells were transfected with YFP alone or together with M1-AQP4-KR or M23-AQP4-KR. (A, left) AQP4 immunoblot after BN/PAGE. Gels that are apart were run separately. (A, right) TIRFM showing OAP formation by M23-AQP4-KR but not by M1-AQP4-KR. (B) Osmotic water permeability measured by YFP fluorescence, as in Fig. 3 B. (C) Relative osmotic water permeabilities (error bars indicate SE, n = 5–8 cells on three cover glasses; *, P < 0.01).

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