![Figure 2. Photobleaching of KR-AQP1. (A) FRT cells coexpressing a YFP chloride sensor (green) and KR-AQP1 short (red) were imaged at 20× magnification before (top) and after (bottom) CALI (exposure to red light for 60 s) in the indicated circular regions (circles). (B) Fluorescence of YFP and KR during CALI (error bars indicate standard error [SE], n = 4). (C) Cell viability assayed after CALI (0.3% Triton X-100 as a positive control). Dead cells were stained green (SYTOX).](https://cdn.rupress.org/rup/content_public/journal/jgp/139/1/10.1085_jgp.201110712/4/jgp_201110712_fig2.jpeg?Expires=1767669835&Signature=D3FG-h5YHWvpysZjLtZ~9YHD~BpmrNZ477bPjxWFE-CvMq6~Zzpol-JFwOuc3E~2q~UE-fgMSttt1GrNN7gcMfPsfodH7mE8XRS2lCWT--bN67augkcc8M2xWH0vGGEyV5xSOTFWog01M9X2sNbBAAGUZP187DpQe87O9ozHtVvUk7xIav30Ttcc-JLEOnPtAk~MNLpxoawYFjqDJ0fuWhK-CXI~UZPD7JxIGJJqNMFf25ZysdHpdlyBt~Nv5bTLEaqjfxlMICfPi5iPN8dpjlsIfXorFsB1BivK8ezIPj75s1tjBy-iZkeZIlRUhkefKHj29xXbp75VG1bggvH0MQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 2.
Photobleaching of KR-AQP1. (A) FRT cells coexpressing a YFP chloride sensor (green) and KR-AQP1 short (red) were imaged at 20× magnification before (top) and after (bottom) CALI (exposure to red light for 60 s) in the indicated circular regions (circles). (B) Fluorescence of YFP and KR during CALI (error bars indicate standard error [SE], n = 4). (C) Cell viability assayed after CALI (0.3% Triton X-100 as a positive control). Dead cells were stained green (SYTOX).
