KR/AQP chimeras. (left) Schematic of a KR/AQP1 chimera with KR at the AQP1 N terminus. (right) Chimeras generated for CALI studies, including three AQP1 N-terminal chimeras of different lengths. Linkers of 17 and 3 amino acids separated KR and full-length AQP1 in KR-AQP1 long and KR-AQP1, respectively. In KR-AQP1 short, the seven C-terminal amino acids of KR were truncated. AQP1-KR and AQP4-KR (M1 and M23) were generated as C-terminal chimeras.