Figure 9.
Figure 9. Effect of Shaker S1–S2 linker replacement on Kv1.2 fluorescence. (A) Representative fluorescence records from a Kv1.2-Shaker S1–S2 linker A291C chimera channel (Kv1.2Sh12L). Traces are shown for depolarizations to potentials as labeled, using the same protocol as outlined in Fig. 2. (B) Mean F-V relationship for Kv1.2Sh12L (triangles) compared with Kv1.2 A291C (circles) and Shaker A359C (squares) as previously shown in Fig. 2. For Kv1.2Sh12L, V1/2 and k values for the Boltzmann fit were −56.0 ± 2.5 mV and 15.5 ± 0.8 mV (n = 7).

Effect of Shaker S1–S2 linker replacement on Kv1.2 fluorescence. (A) Representative fluorescence records from a Kv1.2-Shaker S1–S2 linker A291C chimera channel (Kv1.2Sh12L). Traces are shown for depolarizations to potentials as labeled, using the same protocol as outlined in Fig. 2. (B) Mean F-V relationship for Kv1.2Sh12L (triangles) compared with Kv1.2 A291C (circles) and Shaker A359C (squares) as previously shown in Fig. 2. For Kv1.2Sh12L, V1/2 and k values for the Boltzmann fit were −56.0 ± 2.5 mV and 15.5 ± 0.8 mV (n = 7).

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