Hminx2 RNAi, but not Hminx1 RNAi, inhibited dye loss from the connective glial cell. (A) Photomicrographs of representative glial cells containing 6-CF dye. One pair of glial cells in the connectives was injected with Hminx2 RNAi into the left cell and Hminx1 RNAi into the right cell 2 d before the cells were injected with 6-CF dye, which was allowed to diffuse throughout the cells. The tissue was then slightly stretched to open the innexon channels. The left panel shows the cells at time 0, and the right panel shows them 54 min later. The glial cell injected with Hminx2 RNAi retained substantially more dye than the control glial cell injected with the Hminx1 RNAi. (B) Time course of dye loss from the experiment shown in A. The Hminx2 RNAi–injected cell lost dye at a slower rate than its Hminx1 RNAi–injected neighbor did. A movie of this experiment (Video 1) can be seen in the supplemental material.