Diminished difference between 125 mM and 0 mM Nao at large negative potentials in ΔYY and ΔKESYY truncated Na/K pumps. (A–D) Mean ouabain-sensitive outward Na/K pump currents (normalized as in Fig. 3 D; mean ± SEM of 6–12 oocytes from at least two frogs) at 15 mM [Ko] and 0 mM Nao (open squares) or 125 mM Nao (closed squares) for ΔKESYY (A and C) and ΔYY (B and D) truncated pumps are shown superimposed on the corresponding I-V plots for the parent C113Y (A and B) and RD (C and D) pumps: (A) C113Y (red) and C113Y-ΔKESYY (blue); (B) C113Y (red) and C113Y-ΔYY (olive); (C) RD (green) and RD-ΔKESYY (magenta); (D) RD (green) and RD-ΔYY (orange). Error bars represent SEM.