G45E hemichannels exhibit a larger unitary conductance. Representative examples of patch clamp recordings from inside-out patches containing single WT Cx26 (A), A40V (B), and G45E (C) hemichannels. The single hemichannel currents shown are in response to 8-s voltage ramps between ±70 mV and are leak subtracted (see Materials and methods). In contrast to other hemichannels that generally show inward current rectification in symmetric 140 mM KCl, WT Cx26 shows slight outward rectification. A recording of a single Cx50 hemichannel in the same solutions is included for comparison in A.