Figure 9.
Figure 9. Voltage dependences of the open probabilities of WT/E71A heteromers. (A) Plots of open probability (Po) versus voltage. Diamonds, WT4; squares, WT3E71A1; triangles, WT2E71A2; circles, WT1E71A3; stars, E71A4. Each data point represents the mean ± SD from at least three separate bilayers. The measurements were done in 200 mM KCl, buffered with 10 mM HEPES, pH 7.0, in the cis chamber and 200 mM KCl, buffered with 10 mM succinic acid, pH 4.0, in the trans chamber. (B) Normalized values of the data shown in A for WT4 and WT3E71A1. Values were normalized to the maximum Po value, which was set at Po = 1. Diamonds, WT4; squares, WT3E71A1. The continuous curves are the best fits to Boltzmann functions for WT4 (black) and WT3E71A1 (red).

Voltage dependences of the open probabilities of WT/E71A heteromers. (A) Plots of open probability (Po) versus voltage. Diamonds, WT4; squares, WT3E71A1; triangles, WT2E71A2; circles, WT1E71A3; stars, E71A4. Each data point represents the mean ± SD from at least three separate bilayers. The measurements were done in 200 mM KCl, buffered with 10 mM HEPES, pH 7.0, in the cis chamber and 200 mM KCl, buffered with 10 mM succinic acid, pH 4.0, in the trans chamber. (B) Normalized values of the data shown in A for WT4 and WT3E71A1. Values were normalized to the maximum Po value, which was set at Po = 1. Diamonds, WT4; squares, WT3E71A1. The continuous curves are the best fits to Boltzmann functions for WT4 (black) and WT3E71A1 (red).

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