Figure 4.
![Figure 4. Expression of heteromeric KcsA channels by coupled IVTT. The samples were separated in a 12.5% SDS polyacrylamide gel supplemented with 10 mM KCl. The proteins were labeled with [35S]methionine and visualized by exposure of the dried gel to x-ray film. Lanes 1–5: protein products from plasmid DNAs encoding WT-long and E71A-short in the ratios 4:0, 1:3, 2:2, 3:1, and 0:4. A schematic representation of the distribution of subunits in the heterotetramers is shown (right).](https://cdn.rupress.org/rup/content_public/journal/jgp/135/1/10.1085_jgp.200910305/4/jgp_200910305_gs_fig4.jpeg?Expires=1767799313&Signature=Usik1aIKYF-3D9UusqxSMzz7N-LlG9iYhzBqwWw2wsJzfPLwBtDkLdOdeMJfA7VvNiyaO23zCYDA0d1mfkFxqbQvH6-UYFf20wPbsOXcQGKF6Pp5yCgufj426erFfJZOILD5p2S91My5xa9w6hqGLJPs0yXe5K8N7ptjq-HorUMCF8s6-bPdah-osO9pMs~agqFaGqT7~acUo2oncKeHW~XLBNVaSLU92qFVGm24HZMcwfMmlEeYMwBg7mZJvE-AUoroj2le0QyCNrteeP~BY30jAANQqSsH9QABiICfchsc2KQfBzIuD75vrPhjVo2DFMBJhUITEqR4Es61KfS7nw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Expression of heteromeric KcsA channels by coupled IVTT. The samples were separated in a 12.5% SDS polyacrylamide gel supplemented with 10 mM KCl. The proteins were labeled with [35S]methionine and visualized by exposure of the dried gel to x-ray film. Lanes 1–5: protein products from plasmid DNAs encoding WT-long and E71A-short in the ratios 4:0, 1:3, 2:2, 3:1, and 0:4. A schematic representation of the distribution of subunits in the heterotetramers is shown (right).
