MZB cell defect in the MMTV-Cre;Mib1f/f mice. (A) The splenocytes from the indicated mice were analyzed by flow cytometry for the expression of CD21 and CD23 gated on B220+ cells. MZB and FOB cells were defined as CD21hiCD23lo/− and CD21intCD23hi, respectively. Percentages indicated are mean ± SD from 10 independent experiments. (B) The splenocytes from the MMTV-Cre;Mib1+/f (left) and MMTV-Cre;Mib1f/f (right) mice were stained for CD1d and CD9 gated on B220+ cells. Note the defective CD1dhiCD9hi MZB cells in the mutant mice. Percentages indicated are mean ± SD from 10 independent experiments. (C) FOB cells from the MMTV-Cre;Mib1+/f and MMTV-Cre;Mib1f/f mice were analyzed by flow cytometry for the expression of CD21. A representative of four independent experiments is shown. (D) The splenocytes were stained with antibodies against CD21, CD23, and IgM and were analyzed by flow cytometry for the expression of CD21 and IgM, gated on the CD23− splenocytes for T1 B cells, and gated on the CD23+ splenocytes for T2 B cells. Numbers indicate mean ± SD from five independent experiments. (E) The splenocytes from the indicated mice were analyzed by flow cytometry for the expression of CD23 and IgM gated on B220+AA4.1+ cells. The transitional T2 B cells were defined as CD23+IgMhi. Percentages indicated are mean ± SD from three independent experiments.
