Figure 3.
Figure 3. Reduction of early T cell progenitors in the thymi of MMTV-Cre;Mib1f/f mice. (A) The thymocytes from the littermate control (left) and mutant (right) mice indicated were stained for CD4, CD8, CD44, CD25, CD24, and c-kit. ETPs were identified by CD24 and c-kit expression gated on CD4−CD8−CD25−CD44+ cells. Percentages indicated are mean ± SD from five independent experiments. (B) Leukocytes from the BM (top) and blood (bottom) of the MMTV-Cre;Mib1+/f and MMTV-Cre;Mib1f/f mice were stained for Sca-1 and c-kit gated on lineage-negative cells to identify LSK cells. Numbers indicate representatives of three independent experiments.

Reduction of early T cell progenitors in the thymi of MMTV-Cre;Mib1f/f mice. (A) The thymocytes from the littermate control (left) and mutant (right) mice indicated were stained for CD4, CD8, CD44, CD25, CD24, and c-kit. ETPs were identified by CD24 and c-kit expression gated on CD4−CD8−CD25−CD44+ cells. Percentages indicated are mean ± SD from five independent experiments. (B) Leukocytes from the BM (top) and blood (bottom) of the MMTV-Cre;Mib1+/f and MMTV-Cre;Mib1f/f mice were stained for Sca-1 and c-kit gated on lineage-negative cells to identify LSK cells. Numbers indicate representatives of three independent experiments.

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