![Figure 2. Amplification of Th17 and Th1 cell activity in p55 TNFR−/− mice. LN cells from WT, p55 TNFR−/−, and p75 TNFR−/− mice were taken 14 d after immunization with type II collagen in CFA. (A) LN cells were either unstimulated or stimulated with collagen or with anti-CD3 mAb, and the level of proliferation was determined by [3H]thymidine incorporation. The percentage of CD4+ T cells in the LN was determined by flow cytometry on day 14 after immunization. (B) Levels of IL-17 and IFNγ were determined by ELISA. (C) The proportion of CD4+ cells in the LN producing IL-17 and IFNγ were determined by flow cytometry. Histograms show mean ± SEM (n = 8). *, P < 0.05; **, P < 0.01. Data are representative of two experiments.](https://cdn.rupress.org/rup/content_public/journal/jem/205/11/10.1084_jem.20072707/5/jem_20072707_gs_fig2.jpeg?Expires=1767691955&Signature=OjJnbyuBMOa9tLUflz4cSh64lDvmMQxRnyEhImOdhqT9bv7ZLx46Yu4smy8qNe3o4ITB8dF2JJmE3R6SdR5wXiJ6wfPr19Xkog-9iL-c9WabbKMQMY17QfPUlgYZRvIozjSfcK7q3Q0Sg4X8naJj~dGGOoztc8nsal7n2RftkpzOlty1wmq7~kNc3AYLAm85~vydSBvfYX985d~J2xB9t57xRlitX5CdgO-ME28wtpbGntZgokXuZ62FRtqCxmo-68c8AbakPDUq2p0bbNWPnZXqLbg44k6SIuvzyRk8Wl6ETQk4ybFSFFF2y6WIZoirUBcw~wFuZqlsMzl1giW~WA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 2.
Amplification of Th17 and Th1 cell activity in p55 TNFR−/− mice. LN cells from WT, p55 TNFR−/−, and p75 TNFR−/− mice were taken 14 d after immunization with type II collagen in CFA. (A) LN cells were either unstimulated or stimulated with collagen or with anti-CD3 mAb, and the level of proliferation was determined by [3H]thymidine incorporation. The percentage of CD4+ T cells in the LN was determined by flow cytometry on day 14 after immunization. (B) Levels of IL-17 and IFNγ were determined by ELISA. (C) The proportion of CD4+ cells in the LN producing IL-17 and IFNγ were determined by flow cytometry. Histograms show mean ± SEM (n = 8). *, P < 0.05; **, P < 0.01. Data are representative of two experiments.
