Increased IL-17 and IFNγ production in CIA after blockade of TNFα. DBA/1 mice with CIA were treated with TNFR-Fc or isotype control mAb (100 μg/mouse on alternate days) from the time of disease onset. (A and B) LN cells were taken 10 d after disease onset and levels of IL-17 (A) and IFNγ (B) were determined by ELISA in the supernatants without further stimulation (Nil) or after stimulation with type II collagen (CII) or anti-CD3 mAb (CD3). Data show individual mice (n = 8; *, P < 0.05). (C) Clinical scores were assessed over the 10-d period in TNFR-Fc–treated and control mice. The data are representative of at least three experiments. Error bars show SEM.