Cortical DCs are efficient APC for inducing clonal deletion. Female HY^cd4 D^bo BM was mixed with nontransgenic D^bo BM or CD11c-DTR female BM and transferred into D^bo or WT female recipients, respectively, as controls. Female HY^cd4 D^bo BM was also mixed with male CD11c-DTR BM and transferred into WT females or males and either left untreated or treated with DTx. Activation of Caspase 3 in treated versus untreated T3.70⁺ thymocytes of the indicated group is depicted. Data represent the mean from three to five mice ± SD. The fold change comparing untreated and DTx-treated mice for each group is indicated. P < 0.0001 for the male into female group and P = 0.0242 for the male into male group. ND, not done. Data are representative of four separate experiments.