Male-reactive cells undergoing apoptosis preferentially associate with cortical DCs. (A) Immunofluorescence staining for CD11c in the thymus. Areas of cortex and medulla are indicated. Bar, 250 μm. (B) Thymus tissue sections from mice in Figs. 3 and 5 were analyzed by immunofluorescence for Thy1.2, active Caspase 3, and CD11c. A Thy1.2 and active Caspase 3⁺ colocalized cell immediately adjacent to a CD11c⁺ cell is indicated by arrows. Bar, 5 μm. (C) The frequency of cells associated with CD11c⁺ cells was quantified for the indicated subsets. Data represent the mean from four different males ± SD. P < 0.0001. The frequency of cells associated with F4/80⁺ cells is also depicted. Data are representative of two individual groups of chimeras and one set of immunofluorescence staining.