CCR7 is dispensable for clonal deletion of HY^cd4 thymocytes. (A) The expression of CCR7 on female and male T3.70⁺ thymocytes from HY^cd4 mice on a WT or Bim^o background. (B) BM from female HY^cd4 mice on either WT or CCR7° background was mixed with WT female or male BM and transferred into WT female or male recipients. Activation of Caspase 3 in T3.70⁺ thymocytes of the indicated genotype is depicted. Data represent the mean from four individuals from one experiment ± SD. P = 0.0002 when comparing either WT males and females or CCR7° males and females. The difference between WT males and CCR7° males was not significant. (C) HY^cd4 D^bo BM was mixed with WT or plt/plt female or male BM and transferred into WT or plt/plt female or male mice. The expression of CD69 and PD-1 on T3.70⁺ thymocytes from the indicated group is shown. (D) The activation of Caspase 3 on T3.70⁺ thymocytes. Data depicts the mean ± SD. The fold change is indicated. P = 0.0066. Data are representative of three separate experiments.