Developmental accumulation of CD8α⁺CD4⁻ and CD8α⁻CD4⁺ DCs occurs at day 6 after birth. Splenic cells (10⁶ cells/ tube) from 1-, 2-, 4-, 6-, 8-, and 10-d-old BALB/c neonates were stained with anti-CD11c, anti-CD4, and anti-CD8α antibodies, fixed with 2% formaldehyde, and analyzed by FACS. Expression of CD4 and CD8α was analyzed on cells gated on CD11c. Splenic cells from adult (8-wk-old) BALB/c mice were included as control. Percentages of cells are shown.