Figure 6
Figure 6. Examination of the functional IL-12 responses of developing Th cells. FACS®-sorted naive CD4+ DO11.10 T cells were cultured with OVA peptide and irradiated BALB/c splenocytes in the presence of the primary culture conditions indicated. Cultures were harvested on day 7, washed, and restimulated at 1.25 × 105 T cells/well with OVA peptide and BALB/c splenocytes in the presence of anti–IL-4 mAb (10 μg/ml 11B11) and anti IL-10 (20 μg/ml 2A5) and either IL-12 (10 U/ml) or anti–IL-12 (3 μg/ml TOSH). Supernatants collected after 48 h were analyzed by ELISA for IFN-γ.

Examination of the functional IL-12 responses of developing Th cells. FACS®-sorted naive CD4+ DO11.10 T cells were cultured with OVA peptide and irradiated BALB/c splenocytes in the presence of the primary culture conditions indicated. Cultures were harvested on day 7, washed, and restimulated at 1.25 × 105 T cells/well with OVA peptide and BALB/c splenocytes in the presence of anti–IL-4 mAb (10 μg/ml 11B11) and anti IL-10 (20 μg/ml 2A5) and either IL-12 (10 U/ml) or anti–IL-12 (3 μg/ml TOSH). Supernatants collected after 48 h were analyzed by ELISA for IFN-γ.

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