Figure 3. Activation in the presence of IL-4 and IL-12 results in the maintenance of functional IL-12 responsiveness. FACS®-sorted naive CD4+ DO11.10 T cells were cultured for seven days with OVA peptide and irradiated BALB/c splenocytes under the indicated primary culture conditions. Cultures were harvested on day 7, washed, and restimulated at 1.25 × 105 T cells/well with OVA peptide and BALB/c splenocytes in the presence of the indicated cytokines or anti-cytokine antibodies. Supernatants collected after 48 h were analyzed by ELISA for IFN-γ.
Figure 3

Activation in the presence of IL-4 and IL-12 results in the maintenance of functional IL-12 responsiveness. FACS®-sorted naive CD4+ DO11.10 T cells were cultured for seven days with OVA peptide and irradiated BALB/c splenocytes under the indicated primary culture conditions. Cultures were harvested on day 7, washed, and restimulated at 1.25 × 105 T cells/well with OVA peptide and BALB/c splenocytes in the presence of the indicated cytokines or anti-cytokine antibodies. Supernatants collected after 48 h were analyzed by ELISA for IFN-γ.

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