Figure 4. KIF1A vesicle association and... | Rockefeller University Press

$KIF1A vesicle association and motor dimerization are required for vesicle transport. Comovement of KIF1A with cargo and investigation of the involvement of KIF1A dimerization in cargo transport. (A–D) Stills showing movement of KIF1A_FL (A), KIF1A_ΔPH (B), KIF1A_ΔCC2 (C), or KIF1A_ΔCC3 (D) and NPY vesicles in the axon. Arrowheads mark a moving NPY vesicle colocalizing with KIF1A_FL (A) or KIF1A_ΔCC2 (C). (E) Representative image of a hippocampal neuron coexpressing KIF1A_ΔCC1 and NPY. Blue dotted circles indicate examples of distal tips. Merged image of the boxed region is shown on the right. (F) Schematic depiction of the structure of the KIF1A_ΔCC2-GCN4 motor. (G) Representative image of a hippocampal neuron coexpressing KIF1A_ΔCC2-GCN4 and NPY. Blue dotted circles indicate examples of distal tips. Merged image of the boxed region is shown on the right. (H) Representative image of a hippocampal neuron expressing KIF1A_ΔCC2-GCN4. (I) Quantification of the polarity index of KIF1A_ΔCC2-GCN4 motor (N = 3, n = 38). (J) Quantification of the fraction of static, anterograde, and retrograde NPY traces in control, KIF1A KD, and rescue with KIF1A_ΔCC2-GCN4 (N = 3, n = 35). Data are displayed as mean ± SEM. Unpaired t test, ***, P < 0.001. Scale bars, 1 µm (A–D) and 20 µm (E, G, and H).$

Figure 4.

KIF1A vesicle association and motor dimerization are required for vesicle transport. Comovement of KIF1A with cargo and investigation of the involvement of KIF1A dimerization in cargo transport. (A–D) Stills showing movement of KIF1A_FL (A), KIF1A_ΔPH (B), KIF1A_ΔCC2 (C), or KIF1A_ΔCC3 (D) and NPY vesicles in the axon. Arrowheads mark a moving NPY vesicle colocalizing with KIF1A_FL (A) or KIF1A_ΔCC2 (C). (E) Representative image of a hippocampal neuron coexpressing KIF1A_ΔCC1 and NPY. Blue dotted circles indicate examples of distal tips. Merged image of the boxed region is shown on the right. (F) Schematic depiction of the structure of the KIF1A_ΔCC2-GCN4 motor. (G) Representative image of a hippocampal neuron coexpressing KIF1A_ΔCC2-GCN4 and NPY. Blue dotted circles indicate examples of distal tips. Merged image of the boxed region is shown on the right. (H) Representative image of a hippocampal neuron expressing KIF1A_ΔCC2-GCN4. (I) Quantification of the polarity index of KIF1A_ΔCC2-GCN4 motor (N = 3, n = 38). (J) Quantification of the fraction of static, anterograde, and retrograde NPY traces in control, KIF1A KD, and rescue with KIF1A_ΔCC2-GCN4 (N = 3, n = 35). Data are displayed as mean ± SEM. Unpaired t test, ***, P < 0.001. Scale bars, 1 µm (A–D) and 20 µm (E, G, and H).

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