Figure S4.
Analysis of the localization of TORC1 reporters in the vps11-1 mutant. (A–D) Localization of Kog1 and Ivy1 relative to VT and ET reporters. vps11-1 cells expressing Kog1-mCherry or Ivy1-mScarlet were transformed with VT (Sch9C-term-GFP-Pho8N-term) or ET (FYVE-GFP-Sch9C-term) reporters, grown exponentially at 24 or 30°C in a synthetic medium, and analyzed by fluorescence microscopy. Individual slices are shown. Scale bar, 5 µm. Arrows show colocalizing dots. (E) Quantification of Kog1 or Ivy1 colocalizing with ET dots. Cells (n ≥ 150), Kog1 dots (n ≥ 100), Ivy1 dots (n ≥ 150), and ET dots (n ≥ 200) were quantified by ImageJ. Error bars represent SD of three independent experiments.

Analysis of the localization of TORC1 reporters in the vps11-1 mutant. (A–D) Localization of Kog1 and Ivy1 relative to VT and ET reporters. vps11-1 cells expressing Kog1-mCherry or Ivy1-mScarlet were transformed with VT (Sch9C-term-GFP-Pho8N-term) or ET (FYVE-GFP-Sch9C-term) reporters, grown exponentially at 24 or 30°C in a synthetic medium, and analyzed by fluorescence microscopy. Individual slices are shown. Scale bar, 5 µm. Arrows show colocalizing dots. (E) Quantification of Kog1 or Ivy1 colocalizing with ET dots. Cells (n ≥ 150), Kog1 dots (n ≥ 100), Ivy1 dots (n ≥ 150), and ET dots (n ≥ 200) were quantified by ImageJ. Error bars represent SD of three independent experiments.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal