UNC-40 promotes the synaptic localization of ACR-16 and SDN-1. (A and B) Confocal detection and quantification of ACR-16-wrmScarlet (A) and mNG-SDN-1 (B) fluorescence. (C) Confocal detection and quantification of RFP-UNC-40 driven by the muscle-specific Pmyo-3 promoter. (D) Confocal detection and fluorescent profiles of RFP-UNC-40 and GABA boutons using the SNB-1-GFP marker driven by the Punc-25 promoter. (E) Pearson’s correlation coefficient between RFP-UNC-40 and GABA boutons. Scale bars, 10 µm.