Reducing interkinetochore tension slows release of kinetochore microtubules. (A) Representative images of cells treated with a KIF15 inhibitor (40 µM KIF15-IN-1), Eg5 inhibitor (20 µM BRD9876), or control (no inhibitor). In the plot, each data point represents a single kinetochore pair. Black lines: Mean ± SEM (n = 220 kinetochores for control; n = 40 for KIF15-IN-1; n = 81 for BRD9876; ∼8 pairs measured per cell). Entire titration curves are plotted in Fig. S3, F and G. (B and C) Displacement of the activated kinetochore after 2×INBox recruitment with KIF15 (B; n = 93) or Eg5 (C; n = 74) inhibition, plotted as in Fig. 2, D and E. (D) Waiting time distribution of the released kinetochores (control: median = 1.5 min, n = 41; KIF15-IN-1: median = 2.5 min, n = 37; BRD9876: median = 2.5 min, n = 41). (E) Model for distinct error-correction pathways at merotelic (red) and syntelic (blue) attachments in response to Aurora B activation. *, P < 0.05.
Figure 4.

Reducing interkinetochore tension slows release of kinetochore microtubules. (A) Representative images of cells treated with a KIF15 inhibitor (40 µM KIF15-IN-1), Eg5 inhibitor (20 µM BRD9876), or control (no inhibitor). In the plot, each data point represents a single kinetochore pair. Black lines: Mean ± SEM (n = 220 kinetochores for control; n = 40 for KIF15-IN-1; n = 81 for BRD9876; ∼8 pairs measured per cell). Entire titration curves are plotted in Fig. S3, F and G. (B and C) Displacement of the activated kinetochore after 2×INBox recruitment with KIF15 (B; n = 93) or Eg5 (C; n = 74) inhibition, plotted as in Fig. 2, D and E. (D) Waiting time distribution of the released kinetochores (control: median = 1.5 min, n = 41; KIF15-IN-1: median = 2.5 min, n = 37; BRD9876: median = 2.5 min, n = 41). (E) Model for distinct error-correction pathways at merotelic (red) and syntelic (blue) attachments in response to Aurora B activation. *, P < 0.05.

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