Related to Fig. 4. (A and B) Displacement of the activated kinetochore, plotted as in Fig. 2, D and E, with 20 µM proTAME to delay mitotic exit (2×INBox: n = 54; INBox: n = 56). (C and D) Waiting time of the released kinetochores after recruiting 2×INBox (control: median = 1.5 min, n = 41; proTAME: median = 1.5 min, n = 28) or INBox (control: median = 3.5 min, n = 20; proTAME: median = 3.0 min, n = 23). (E) The fraction of release vs. depolymerization events after recruiting 2×INBox or INBox. Differences in the presence or absence of proTAME (C–E) are not statistically significant (P > 0.05; mean ± SEM). (F and G) Interkinetochore distances at varying concentrations of rigor inhibitors for KIF15 (KIF15-IN-1: IC₅₀ = 21 µM) or Eg5 (BRD9876: IC₅₀ = 2 µM). Open triangles represent the concentrations used for 2×INBox recruitment assays in Fig. 4, B and C (KIF15-IN-1: 40 µM; KIF15-IN-1: 20 µM). (H) The fraction of release vs. depolymerization events from Fig. 4, B and C (control: 87 ± 5% mean ± SEM, n = 47; KIF15-IN-1: 84 ± 6% mean ± SEM, n = 44; BRD9876: 95 ± 3% mean ± SEM, n = 43).