Neurite tip accumulation of endo-lysosomal organelles in the unc-16(V72Q) mutant requires kinesin-1 activity but not MAP kinase signalling, and N-terminal UNC-16 deletions mimic the unc-16(V72Q) mutant. (A) Domain organization of the N-terminal UNC-16 region with the putative binding sites for UNC-116/KHC and JNK-1 delineated by dashed lines. (B and D) Fluorescence intensity profiles (mean ± SEM) along the ALM neurite in L4 animals expressing a transgene-encoded marker for lysosomes (CTNS-1::mKate2), late endosomes (mKate2::RAB-7), or early endosomes (mKate2::RAB-5) in touch receptor neurons. n denotes the number of neurites examined (1 per animal). (C and E) Integrated fluorescence intensity [mean ± SEM, normalized to unc-16(V72Q)] in the ALM cell body and the last 20 µm of the distal neurite (neurite tip). The number of neurites examined in C and E corresponds to the number n in B and D, respectively. Statistical significance was determined by the Mann-Whitney test. ****P < 0.0001; **P < 0.01; *P < 0.05; ns = not significant, P > 0.05. (F and G) Left: Fluorescence intensity profiles (mean ± SEM) in the ALM neurite, as described for B and D. Right: Integrated fluorescence intensity (mean ± SEM, normalized to unc-16(V72Q) for F and to the control for G) in the cell body and at the neurite tip. The number of neurites examined corresponds to the number n in the fluorescence intensity profiles on the left. Statistical significance (control versus mutants and unc-16(V72Q) versus unc-16(V72Q); jnk-1(gk7)) was determined by the Mann-Whitney test for F and by ANOVA on ranks (Kruskal-Wallis nonparametric test) followed by Dunn’s multiple comparison test for G. ****P < 0.0001; *P < 0.05; ns = not significant, P > 0.05.