Loss of RTKN-1 impairs basolateral recycling. (A and A′) Confocal images showing the subcellular localization of CIE recycling cargo hTAC-GFP in intestinal cells. Box-and-whisker plots (n = 18 cells): 10–90 percentile; dots, outliers; red midline, median of WT; boundaries, quartiles. ***, P < 0.001 by Mann-Whitney test. (B–C′) Confocal images showing the colocalization between hTAC-GFP and mCherry-tagged RAB-5 or RME-1. Arrowheads indicate structures labeled by both GFP and mCherry. DAPI channel (blue color) indicates broad-spectrum intestinal autofluorescence. Pearson's correlation coefficients were calculated; error bars are 95% CIs (n = 12 animals). ***, P < 0.001 by Mann-Whitney test. (D and D′) Live cell fluorescence images showing hTAC-GFP–positive endosomal dynamics. A total of eight animals of each genotype were sampled. (E–G′) Confocal images showing the localization of early endosome marker GFP-RAB-5, sorting endosome marker GFP-RAB-10, and recycling endosome marker GFP-RME-1 in intestinal cells. Box-and-whisker plots (n = 18 cells): 10–90th percentile; dots, outliers; red midline, median of WT; boundaries, quartiles. ***, P < 0.001 by Mann-Whitney test. The black asterisks in the image panels indicate intestinal lumen. Scale bars, 10 µm. MC, mCherry.