Loss of RTKN-1 affects basolateral endocytic recycling. (A–C′) Confocal images showing the localization of CIE recycling cargo hTAC-GFP, clathrin-dependent nonrecycling cargo GFP-CD4-LL, and CDE recycling cargo hTfR-GFP in intestinal cells. Box-and-whisker plots (n = 18 cells): 10–90th percentile; dots, outliers; red midline, median of WT; boundaries, quartiles. ***, P < 0.001 by Mann-Whitney test. (D–E′) Confocal images showing colocalization between hTfR-GFP and early endosome marker mCherry-RAB-5 or recycling endosome marker mCherry-RME-1. DAPI channel (blue color) indicates broad-spectrum intestinal autofluorescence. Pearson's correlation coefficients were calculated; error bars are 95% CIs (n = 12 animals). ***, P < 0.001 by Mann-Whitney test. (F–H′) Confocal images showing localization of clathrin-dependent retrograde cargo MIG-14-GFP, clathrin-dependent nonrecycling cargo GFP-CD4-LL, and apical recycling cargo LRP-1-GFP. Box-and-whisker plots (n = 18 cells): 10–90th percentile; dots, outliers; red midline, median of WT; boundaries, quartiles. No significant difference by Mann-Whitney test. (I–J′) Confocal images showing subcellular localization of recycling endosome markers ARF-6-GFP and SDPN-1-GFP. Box-and-whisker plots (n = 18 cells): 10–90th percentile; dots, outliers; red midline, median of WT; boundaries, quartiles. ***, P < 0.001 by Mann-Whitney test. The white arrowheads indicate structures labeled by both GFP and mCherry. The asterisks in the image panels indicate intestinal lumen. Scale bars, 10 µm. Ctrl, control; MC, mCherry.
Figure S3.

Loss of RTKN-1 affects basolateral endocytic recycling. (A–C′) Confocal images showing the localization of CIE recycling cargo hTAC-GFP, clathrin-dependent nonrecycling cargo GFP-CD4-LL, and CDE recycling cargo hTfR-GFP in intestinal cells. Box-and-whisker plots (n = 18 cells): 10–90th percentile; dots, outliers; red midline, median of WT; boundaries, quartiles. ***, P < 0.001 by Mann-Whitney test. (D–E′) Confocal images showing colocalization between hTfR-GFP and early endosome marker mCherry-RAB-5 or recycling endosome marker mCherry-RME-1. DAPI channel (blue color) indicates broad-spectrum intestinal autofluorescence. Pearson's correlation coefficients were calculated; error bars are 95% CIs (n = 12 animals). ***, P < 0.001 by Mann-Whitney test. (F–H′) Confocal images showing localization of clathrin-dependent retrograde cargo MIG-14-GFP, clathrin-dependent nonrecycling cargo GFP-CD4-LL, and apical recycling cargo LRP-1-GFP. Box-and-whisker plots (n = 18 cells): 10–90th percentile; dots, outliers; red midline, median of WT; boundaries, quartiles. No significant difference by Mann-Whitney test. (I–J′) Confocal images showing subcellular localization of recycling endosome markers ARF-6-GFP and SDPN-1-GFP. Box-and-whisker plots (n = 18 cells): 10–90th percentile; dots, outliers; red midline, median of WT; boundaries, quartiles. ***, P < 0.001 by Mann-Whitney test. The white arrowheads indicate structures labeled by both GFP and mCherry. The asterisks in the image panels indicate intestinal lumen. Scale bars, 10 µm. Ctrl, control; MC, mCherry.

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