RTKN-1 is localized in recycling endosomes. (A and A′) Confocal images showing colocalization between RTKN-1-mCherry and recycling endosome markers. (B–C′) Confocal images showing colocalization between GFP-tagged RTKN-1 or RTKN-1-PH and endosome markers. In these panels, the DAPI channel (blue color) indicates broad-spectrum intestinal autofluorescence. Pearson's correlation coefficients were calculated; error bars are 95% CIs (n = 12 animals). ***, P < 0.001 by Mann-Whitney test. (D–I) mRNA level measurement showing RNAi efficiency of phosphatidylinositol kinases. The white arrowheads indicate structures labeled by both GFP and mCherry. Scale bars, 10 µm. Ctrl, control; MC, mCherry; Tb, Tubby.
Figure S2.

RTKN-1 is localized in recycling endosomes. (A and A′) Confocal images showing colocalization between RTKN-1-mCherry and recycling endosome markers. (B–C′) Confocal images showing colocalization between GFP-tagged RTKN-1 or RTKN-1-PH and endosome markers. In these panels, the DAPI channel (blue color) indicates broad-spectrum intestinal autofluorescence. Pearson's correlation coefficients were calculated; error bars are 95% CIs (n = 12 animals). ***, P < 0.001 by Mann-Whitney test. (D–I) mRNA level measurement showing RNAi efficiency of phosphatidylinositol kinases. The white arrowheads indicate structures labeled by both GFP and mCherry. Scale bars, 10 µm. Ctrl, control; MC, mCherry; Tb, Tubby.

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