RTKN-1 resides in basolateral recycling endosome. (A and A′) Confocal images showing colocalization between RTKN-1-mCherry and organelle markers. Arrowheads indicate structures labeled by both GFP and mCherry. DAPI channel (blue color) indicates broad-spectrum intestinal autofluorescence. Pearson's correlation coefficients were calculated; error bars are 95% CIs (n = 12 animals). (B and B′) Confocal images showing RTKN-1-GFP distribution upon knock down of phosphatidylinositol kinases. Box-and-whisker plots (n = 18 cells): 10–90th percentile; dots, outliers; red midline, median of WT; boundaries, quartiles **, P < 0.01; ***, P < 0.001, by one-way ANOVA followed by Dunn’s post hoc multiple comparison test. Scale bars, 10 µm. Ctrl, control; MC, mCherry.
Figure 2.

RTKN-1 resides in basolateral recycling endosome. (A and A′) Confocal images showing colocalization between RTKN-1-mCherry and organelle markers. Arrowheads indicate structures labeled by both GFP and mCherry. DAPI channel (blue color) indicates broad-spectrum intestinal autofluorescence. Pearson's correlation coefficients were calculated; error bars are 95% CIs (n = 12 animals). (B and B′) Confocal images showing RTKN-1-GFP distribution upon knock down of phosphatidylinositol kinases. Box-and-whisker plots (n = 18 cells): 10–90th percentile; dots, outliers; red midline, median of WT; boundaries, quartiles **, P < 0.01; ***, P < 0.001, by one-way ANOVA followed by Dunn’s post hoc multiple comparison test. Scale bars, 10 µm. Ctrl, control; MC, mCherry.

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