RTKN-1 could play a role during development, and loss of RTKN-1 impairs actin integrity. (A–A′′) Measurement of growth time from the L1 larval stage to the young adult stage. (B) Differential interference contrast microscopy showing WT and mutant animals. Scale bars, 50 µm. (C and C′) Confocal images showing Alexa Fluor 488–phalloidin staining in the fixed intestinal cells. White asterisks in the middle focal panels indicate intestinal lumen. Box-and-whisker plots (n = 18 cells): 10–90th percentile; dots, outliers; red midline, median of WT; boundaries, quartiles. ***, P < 0.001 by Mann-Whitney test. (D) Loss of RTKN-1 did not interfere with expression of intestinal actin. (E and E′) Confocal images showing the colocalization between GFP-Utrophin-CH and endogenous RTKN-1. The white arrowheads indicate structures labeled by both GFP and mCherry. Pearson's correlation coefficients were calculated; error bars are 95% CIs (n = 12 animals). Scale bars, 10 µm.
Figure S1.

RTKN-1 could play a role during development, and loss of RTKN-1 impairs actin integrity. (A–A′′) Measurement of growth time from the L1 larval stage to the young adult stage. (B) Differential interference contrast microscopy showing WT and mutant animals. Scale bars, 50 µm. (C and C′) Confocal images showing Alexa Fluor 488–phalloidin staining in the fixed intestinal cells. White asterisks in the middle focal panels indicate intestinal lumen. Box-and-whisker plots (n = 18 cells): 10–90th percentile; dots, outliers; red midline, median of WT; boundaries, quartiles. ***, P < 0.001 by Mann-Whitney test. (D) Loss of RTKN-1 did not interfere with expression of intestinal actin. (E and E′) Confocal images showing the colocalization between GFP-Utrophin-CH and endogenous RTKN-1. The white arrowheads indicate structures labeled by both GFP and mCherry. Pearson's correlation coefficients were calculated; error bars are 95% CIs (n = 12 animals). Scale bars, 10 µm.

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