Electrophysiological analyses of Dscam2 mutant NMJs. (A) Quantification of evoked junctional potentials corrected for nonlinear summation (EJP’) in control (black), Dscam2^null (red), Dscam2A (blue), and Dscam2B (magenta) at 0.5, 0.75, 1.5, and 3 mM of [Ca²⁺]_o. n = 8–10. (B) Variance-amplitude plots of control (black), Dscam2^null (red), Dscam2A (blue), and Dscam2B (magenta) with lines of best fit generated by nonlinear (quadratic) regression. Error bar crosses represent SEM for trial-to-trial EJP’ variability (vertical error bars) and EJP’ amplitude (horizontal error bars), with corresponding nearby numbers indicating [Ca²⁺]_o.n = 7–10. (C) Representative frequency histograms for mEJP amplitude from individual NMJs. (D–F) Quantification of muscle resting membrane potential (RMP; D), EJP width at half maximum (WAHM; E), and EJP rise time (F). n indicated in graph. Data shown as mean ± SEM; two-way ANOVA; groups within conditions compared with Sidak’s post-test. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001 for all panels.