Dscam2 single isoform splice trap expression patterns in larvae and embryos.(A and A’) Co-labeling of HRP (red), phalloidin (blue), and Dscam2B>CD8GFP (green) in a single peripheral body-wall hemi-segment of a filleted third-instar larva. NMJs (red) and sensory neurons (white arrowheads) are seen in internal musculature (A) and external musculature (A’). Scale bar, 50 µm. (B–F’) Representative images of Dscam2A>CD8GFP (B–F) and Dscam2B>CD8GFP (B’–F’) expression pattern in embryos aged 12–24 h AEL. Red arrowheads indicate central nervous system neurons, and yellow arrowheads indicate PNS neurons. Scale bar, 100 µm. (G–J’) Co-labeling of HRP (gray) and Dscam2A>CD8GFP (green, G–H’) and Dscam2B>CD8GFP (green, I–J’) of filleted stage 16 embryos. Arrowheads as in B–F'. Scale bars, 20 µm.
Figure S1.

Dscam2 single isoform splice trap expression patterns in larvae and embryos. (A and A’) Co-labeling of HRP (red), phalloidin (blue), and Dscam2B>CD8GFP (green) in a single peripheral body-wall hemi-segment of a filleted third-instar larva. NMJs (red) and sensory neurons (white arrowheads) are seen in internal musculature (A) and external musculature (A’). Scale bar, 50 µm. (B–F’) Representative images of Dscam2A>CD8GFP (B–F) and Dscam2B>CD8GFP (B’–F’) expression pattern in embryos aged 12–24 h AEL. Red arrowheads indicate central nervous system neurons, and yellow arrowheads indicate PNS neurons. Scale bar, 100 µm. (G–J’) Co-labeling of HRP (gray) and Dscam2A>CD8GFP (green, G–H’) and Dscam2B>CD8GFP (green, I–J’) of filleted stage 16 embryos. Arrowheads as in B–F'. Scale bars, 20 µm.

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