Examples of structurally aberrant centrioles.(A) Electron micrographs of aberrant centrioles from HT1080 cells, fixed ∼3 h after they exit from 3-h-long mitosis. Consecutive 80-nm-thick serial sections (S1–4) are shown. One section of each centriole is presented in Fig. 3 F. (B) Examples of expanded aberrant centrioles fixed 48 h after the exit from ∼3-h-long mitosis. (C). Examples of over-elongated and structurally aberrant centrioles from cycling MDA-MB-435 cell line. Cells were expanded ∼4.2×, and centrioles were labeled for acetylated tubulin and distal appendage protein Cep164. Left: A cell showing over-elongated centriole. Right: More examples of aberrant centrioles from the same cell population. Overly long and aberrant centrioles often lack or have an irregular or diminished Cep164 signal. Scale bars, 0.2 µm in A; 20 µm for low magnification in C; 2 µm for B and for the insert and the right panel in C.
Figure S2.

Examples of structurally aberrant centrioles. (A) Electron micrographs of aberrant centrioles from HT1080 cells, fixed ∼3 h after they exit from 3-h-long mitosis. Consecutive 80-nm-thick serial sections (S1–4) are shown. One section of each centriole is presented in Fig. 3 F. (B) Examples of expanded aberrant centrioles fixed 48 h after the exit from ∼3-h-long mitosis. (C). Examples of over-elongated and structurally aberrant centrioles from cycling MDA-MB-435 cell line. Cells were expanded ∼4.2×, and centrioles were labeled for acetylated tubulin and distal appendage protein Cep164. Left: A cell showing over-elongated centriole. Right: More examples of aberrant centrioles from the same cell population. Overly long and aberrant centrioles often lack or have an irregular or diminished Cep164 signal. Scale bars, 0.2 µm in A; 20 µm for low magnification in C; 2 µm for B and for the insert and the right panel in C.

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