Figure 1.
EXC-9 is localized to the apical luminal surface of the excretory canals by intermediate filament protein EXC-2. (a) Layout of C. elegans excretory canals and canal cell body within the animal. (b) Diagram of EXC-9 domains. (c and d) Comparison of maximum canal cyst size (c) and mean canal length ± SD (d) for different alleles of exc-9; n > 50. ***, P < 0.001, calculated via one-way ANOVA. (e–e’’’’) Collocation of GFP::EXC-9 and labeled intermediate filament mKate2::IFA-4 at the canal apical surface. GFP::EXC-9 expression at canal lumen and cytoplasm (e); mKate2::IFA-4 (e’); merge (e’’); DIC micrograph of same section of canal (arrows indicate luminal surface, e’’’); and fluorescence intensity profiles of EXC-9 (green) and IFA-4 (red) along white line in panel e’’’ (e’’’’). n = 30 animals examined. (f and f’) The qpIs125(exc-9::gfp) allele allows cysts to form but is still retained apically (f); fluorescence intensity profile of EXC-9::GFP along white line in panel f (f’). n = 30 animals examined. (g–j’) Expression of GFP::EXC-9 in homozygous mutants affecting canal apical structure. Fluorescence intensity profile along white lines for each mutant (g’–j’); exc-2− (n = 50, g); ifa-4− (n = 25, h);sma-1− (n = 25, i); and exc-1− (n = 25, j). (k–k’’’) Co-expression of mKate2::EXC-9 and GFP::EXC-2. mKate2::EXC-9 (k); GFP::EXC-2 (k’); merge (k’’); and fluorescence intensity profiles of EXC-9 (green) and IFA-4 (red) along white line in panel k’’ (k’’’). All scale bars, 5 µm.

EXC-9 is localized to the apical luminal surface of the excretory canals by intermediate filament protein EXC-2. (a) Layout of C. elegans excretory canals and canal cell body within the animal. (b) Diagram of EXC-9 domains. (c and d) Comparison of maximum canal cyst size (c) and mean canal length ± SD (d) for different alleles of exc-9; n > 50. ***, P < 0.001, calculated via one-way ANOVA. (e–e’’’’) Collocation of GFP::EXC-9 and labeled intermediate filament mKate2::IFA-4 at the canal apical surface. GFP::EXC-9 expression at canal lumen and cytoplasm (e); mKate2::IFA-4 (e’); merge (e’’); DIC micrograph of same section of canal (arrows indicate luminal surface, e’’’); and fluorescence intensity profiles of EXC-9 (green) and IFA-4 (red) along white line in panel e’’’ (e’’’’). n = 30 animals examined. (f and f’) The qpIs125(exc-9::gfp) allele allows cysts to form but is still retained apically (f); fluorescence intensity profile of EXC-9::GFP along white line in panel f (f’). n = 30 animals examined. (g–j’) Expression of GFP::EXC-9 in homozygous mutants affecting canal apical structure. Fluorescence intensity profile along white lines for each mutant (g’–j’); exc-2− (n = 50, g); ifa-4− (n = 25, h);sma-1− (n = 25, i); and exc-1− (n = 25, j). (k–k’’’) Co-expression of mKate2::EXC-9 and GFP::EXC-2. mKate2::EXC-9 (k); GFP::EXC-2 (k’); merge (k’’); and fluorescence intensity profiles of EXC-9 (green) and IFA-4 (red) along white line in panel k’’ (k’’’). All scale bars, 5 µm.

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