Mitochondrial transport is not altered by CMT-associated KIF5A^R280H. (A) Kymograph of mitochondrial transport in axons of control (Ctrl), WT KIF5A-expressing, and KIF5A^R280H-expressing DRG neurons. Scale bars: 6 µm and 15 s. (B) Fraction of mitochondria spent in stationary, anterograde, or retrograde phase in control, WT KIF5A-expressing, and KIF5A^R280H-expressing DRGs. (C and D) Average run length and velocity of mitochondrial in axons of control, WT KIF5A-expressing, and KIF5A^R280H-expressing DRGs in C anterograde and D retrograde transport. Data represent mean ± SEM. Data from B–D were analyzed from ∼115 mitochondria from 10 axons across three independent microfluidic experiments.