Flow cytometry analysis of immature and mature wt and hem1 ^−/− DCs. (A) Flow cytometry profiles of MHC II– and Cd11c-stained immature (left) or mature (right) WT or hem1^−/− DCs. (B) Flow cytometry histograms of immature (light colors) and mature (dark colors) of WT (top) or hem1^−/− (bottom) DCs stained for CD80, CD86, or CD40, respectively. (C) Flow cytometry histograms of three biological replicates for Lifeact-eGFP–expressing mature WT and hem1^−/− DCs.