SEPT2/6/7 does not interact with DIC. (A) Coomassie-stained gel (top) and Western blot (bottom; anti-His) show results from pull-down assays of purified recombinant His-SEPT2/6/7 with GST (control) or GST-tagged DIC(1–108), DIC(108–268), and DIC(1–268). (B) Bar graph shows ratio (mean ± SEM) of His-SEPT2/6/7 to GST and GST-DIC fragments from three independent experiments.